First report of Eggplant mottled dwarf virus in mainland Spain.
Eggplant mottled dwarf virus (EMDV) is widespread in most Mediterranean countries, but in Spain the virus has only been described in the Canary Islands. In June 2005, vein-clearing, leaf-crinkling and stunting symptoms were observed in approximately 5% of cucumber plants, grown in 3 glasshouses in northeastern Spain. The virus was mechanically transmitted to Gomphrena globosa, Chenopodium quinoa and Petunia hybrida, producing local lesions 5-10 days after inoculation. Mechanical transmission to cucumber, Nicotiana glutinosa, N. benthamiana, N. tabacum cv. Xanthi and aubergine sporadically induced local lesions and finally showed symptoms of systemic infection. No infection was found with Capsicum annuum, Datura stramonium and Lycopersicon esculentum. Field plants with symptoms and experimental plants mechanically infected under glasshouse conditions were analysed by ELISA using a polyclonal antiserum (As-0836, DSMZ). Extracts giving optical density values at least fivefold higher than equivalent healthy controls were considered positive. Serological assays also confirmed the presence of EMDV in aubergine leaf samples collected in the same geographical area showing mottling, vein-clearing and leaf deformation. Sap extracts from infected aubergine samples gave a similar host range of infection to sap extracts from infected cucumber. This is thought to be the first report of EMDV in the Iberian Peninsula and the first report of EMDV infecting cucumber and aubergine in Spain.