Biological and molecular characterization of Zucchini yellow mosaic virus from naturally infected bottle gourd.
During a survey of commercial fields of bottle gourd (Lagenaria siceraria) in Pune, Maharashtra, India, in kharif 2003, plants exhibiting severe mosaic, interveinal chlorosis, and leaf deformation were observed that resulted in fern-leaf appearance and severe fruit distortion in approximately 70% of the plants. The virus isolate was identified as Zucchini yellow mosaic virus (ZYMV) by biological and serological techniques, electron microscopy and RT-PCR. Nucleotide sequencing of the cloned PCR product showed that it was 497 bp long. Sequence analysis indicated that it contained C-terminal part of coat protein region and 3′ untranslated region (UTR). The C-terminal coat protein region showed 100% sequence identity with most of the isolates of ZYMV while 3′UTR region shared sequence identity in the range of 86.8% to 99.5% with different isolates of ZYMV, indicating that the virus infecting bottle gourd is an isolate of ZYMV.