Comparative biological and serological properties of Watermelon mosaic virus 2 and Zucchini yellow mosaic virus.
Two pumpkin viruses, Watermelon mosaic virus 2 (WMV-2) and Zucchini yellow mosaic virus (ZYMV), were evaluated for their host range, aphid transmissibility and serological features. It was found that host ranges of WMV-2 and ZYMV were almost similar with few exceptions. ZYMV-infected Luffa acutangula (ribbed gourd) and Gomphrena globosa (button flower) showed latent symptoms and necrotic local lesion, respectively, whereas WMV-2-infected L. acutangula and G. globosa did not produce any symptoms. Both WMV-2 and ZYMV were transmitted by green peach aphid (Myzus persicae) and bean aphid (Aphis craccivora), showing a similar transmissibility. Serological feature between the two viruses was determined by indirect enzyme-linked immunosorbent assay (I-ELISA) using polyclonal antisera against the WMV-2 and ZYMV. The antiserum against WMV-2 reacted not only with WMV-2 but also with ZYMV in I-ELISA, whereas the antiserum against ZYMV reacted only with ZYMV.