Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers.
Genetic relationship of 12 species of section Strobus was analysed with ISSR markers. A total of 117 loci were detected with 12 ISSR primers. The percentage of polymorphic bands varied from 5.93 to 19.92%. Pinus pumila had the highest levels of genetic differentiation and P. flexilis had the lowest. Total genetic diversity (HT) of 12 species in section Strobus was 26.21%, of which intraspecific genetic diversity (HS) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group included P. griffithii [P. wallichiana], P. armandii, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola and P. wangii. The second group included P. albicaulis, P. pumila, P. flexilis, P. sibirica and P. koraiensis.