Inhibition of growth of some seed-borne fungal pathogens by extract from Bacillus subtilis.
The effect of B. subtilis (isolated from barley seed) and its extract on the growth of 13 seedborne fungal pathogens was investigated in vitro. The pathogens used were: Alternaria brassicae, Alternaria brassicicola, Alternaria dauci, Alternaria linicola, Alternaria radicina, Ascochyta fabae, Colletotrichum lini, Fusarium culmorum, F. moniliforme [Gibberella moniliformis], Macrophomina phaseolina, Pyrenophora graminea, P. teres and Stemphylium botryosum [Pleospora herbarum]. The mean percentage inhibition of B. subtilis on the fungi (in terms of fungal dry weight) was 43.0, 37.9, 42.7, 38.5, 36.2, 58.3, 52.8, 54.5, 55.9, 47.6, 45.7, 40.0 and 58.1%, respectively. Bacterium extract containing fengymycin inibited fungal growth in potato dextrose agar. Bacterial slurry was non toxic to barley, maize, wheat, mungbean and soyabean seeds in seed germination tests.