Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Characterization of four isolates of Indian citrus ringspot virus.

Abstract

The 1283 nucleotides of the coat protein of four isolates of Indian citrus ringspot virus, ICRSV-DI, ICRSV-Ab, ICRSV-Ah and ICRSV-Pu, were cloned in pGEM-T easy vector and sequenced. Analysis of nucleotide and deduced amino acid sequences showed variation near the N terminus and conservation in the core region. Sequence analysis and the phylogenetic tree of both nucleotide and amino acid sequences showed that isolates ICRSV-Ab and ICRSV-DI shared close relationship with ICRSV-D (an isolate from Delhi sequenced earlier) and formed one cluster, while isolates ICRSV-Ah and ICRSV-Pu formed another cluster, and were considered as different strains of the virus. The information on ICRSV coat protein gene will be useful in developing pathogen mediated resistance.