Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Alfalfa seed microflora and some characteristics of obtained isolates.

Abstract

In a laboratory experiment, streptomycin completely inhibited the bacteria in seeds of lucerne cultivars Banat and Osijek, whereas Previcur [prothiocarb] was less effective. The germination of seeds untreated with fungicides was 85% for Banat and up to 90% for Osijek. However, the germination of treated seeds of both cultivars was poor. The degree of contamination of seeds of both cultivars by bacteria and fungi reached 71.6-76.6%. Two fungi were isolated from the contaminated seeds: Stemphylium botryosum [Pleospora herbarum] and Phoma medicaginis. The average size of conidia of S. botryosum from potato dextrose agar was 13.5×9.4 µm. The size of pycnidiospores of P. medicaginis was 6.8×3.7 µm. On potato dextrose agar and Sabouraud maltose agar, the fungal colonies were olive green to almost black with cottony margins. Abundant growth of S. botryosum mycelia was observed on potato dextrose agar. The highest number of spores was produced at 21°C on carrot agar (average of 19.9) and Sabouraud maltose agar (up to 22.3×104/ml).