Random amplified polymorphic DNA in four species of fruit flies (Diptera: Tephritidae) distributed commonly in Shaanxi Province, China.
DNA polymorphisms in 4 species of Tephritidae, i.e. Bactrocera citri, B. tau, B. scutellaris and Dacus trimacula, distributed commonly in Shaanxi Province, China, were studied by means of random amplified polymorphic DNA (RAPD). The results showed that only 5 primers (S61, S107, S126, S275, S1142) could be used to amplify DNA and to obtain stable and clear DNA bands in all of tested 31 primers. B. tau and B. scutellaris as well as B. scutellaris and D. trimacula could be distinguished from each other by primer S126. The phylogenetic tree constructed by UPGMA was consistent with the conventional phylogenetic relationship among these species.