Association of mycoflora with the seeds of Rusty shield bearer (Peltophorum ferrugineum) from Uttaranchal.
An investigation was undertaken to determine the prevalence and association of seedborne fungi with the seeds of Rusty shield bearer (P. ferrugineum [P. pterocarpum]). Physiologically mature pods of Rusty shield bearer collected from Uttaranchal, India, were sun-dried, and seeds were extracted manually by breaking individual pods by hammer. Seedborne fungi associated with forest tree seeds were determined by the incubation method. The preliminary identification of the fungi was done based on the sporulation of conidial characters, either sexual or asexual spore or fruiting structure as apparent on the seed in blotter method under stereonocular microscope. The identification of the fungi in agar plate method was done based on the colony characters developed on the agar medium and the morphology of the individual fungal species. Seeds of Rusty shield bearer yielded a total of 8 fungal species: Aspergillus flavus, A. fumigatus, A. niger, A. ustus, A. versicolor, Fusarium sp., Penicillium aurantiogriseum and Rhizopus stolonifer. The fungi encountered in blotter method were Aspergillus niger, Fusarium sp., Penicillium aurantiogriseum and Rhizopus stolonifer. The agar plate method yielded Aspergillus flavus, A. fumigatus, A. ustus, A. versicolor, Fusarium sp. and Penicillium aurantiogriseum. Generally, the maximum incidence of Penicillium aurantiogriseum (27%), followed by A. fumigatus was observed among all the fungi. The rest of the fungi isolated above, except A. fumigatus, constitute first host record from India and the world as well. Agar plate method yielded more number of fungi compared to blotter method.