Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in Sri Lanka using FTATM papers.
The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. In this method, DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time consuming. A study was conducted to investigate the use of FTA paper (Whatman BioScience) for DNA extraction and the use of radioactive probe in conjunction with tissue blot procedure to detect phytoplasmas in sugarcane leaf samples (collected in Sri Lanka) exhibiting symptoms of grassy shoot and white leaf. Bermuda grass leaf samples showing symptoms of bermuda grass white leaf were also included. The FTA paper was a rapid and cost-effective method in detecting sugarcane phytoplasma compared to the enrichment method.