Methodology for the artificial inoculation with Ceratocystis sp. for the control of Spathodea campanulata Beauv.
Several trees of Spathodea campanulata, a weed in coffee plantations, were inoculated with Ceratocystis in Cuba. Inoculation was carried out on trees with trunk diameters of 5-20, 21-40, 41-60 and greater than 60 cm, using a mechanical borer impregnated with mycelia and reproductive structures. The incubation period of the fungus was evaluated on the basis of external and internal symptoms. Trees with the smallest trunk diameter died first, with fungal incubation periods of 22-33 days. It is concluded that this method could be used for weed control.