Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Linkage map based on AFLP and RAPD markers in Japanese black pine.

Abstract

Macrogametophytes derived from the Japanese black pine (Pinus thunbergii) seeds resistant to pine needle gall midge (PGM, Thecodiplosis japonensis) were analysed using amplified fragment length polymorphism (AFLP). A total of 244 segregating loci were detected among 71 macrogametophytes. Combining the AFLP results with previously reported segregation data for 127 RAPD markers, 157 AFLP and 50 RAPD markers with confirmed map positions were assigned to 20 linkage groups and 3 pairs covering 2088.1 cM with an average distance of 11.3 cM. The total map distance covers about 77.1-78.4% of the total genome, estimated to be approximately 2665-2719 cM. Thus, using AFLP markers, the previous RAPD map of P. thunbergii was improved in terms of average distance between markers, total map distance, and coverage of the genome. Three RAPD markers linked to a gene associated with resistance to PGM were also located on this map.