Molecular test to show the presence of mildew (Plasmopara halstedii) in sunflower seeds.
A molecular test was developed to determine the presence of sunflower downy mildew, caused by Plasmopara halstedii, in sunflower seed samples. Several extraction methods were compared to improve the quality and yield of the scanty DNA of the fungus. Both whole and hulled seeds of contaminated samples were analysed by PCR, using P. halstedii-specific primers. The study demonstrated that the DNA of the pathogen is always detected, notably in shell fraction. These data are important for the development of a diagnostic kit.