Invasive Species Compendium

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Abstract

Induction of cytoplasmic male sterility in the seed progeny derived from artificially-synthesized interspecific chimera in Brassica.

Abstract

We produced artificial interspecific chimaeras by in vitro grafting, and obtained cytoplasmic male sterile (CMS) variants in the seed progenies derived from backcrossing the chimaera with one of the mother plants, Brassica campestris cv. Komatsuna. The induced CMS was stably inherited by crossing with Komatsuna, not with cabbage cv. Ruby Ball. The nuclear component of CMS was a complete Komatsuna type in morphology, chromosome number (2n = 20) and Southern blot using ribosomal 17S RNA gene as a probe. PCR analysis by using mitochondrial atpA primer showed the complete Ruby Ball type, suggesting nuclear-cytoplasmic exchange. However, Southern blot patterns were different among those of the CMS and both parents by using atpA. Recombination or some unknown change is supposed in the mitochondrial genome via the processes of synthesis and propagation of the chimaeras.