Invasive Species Compendium

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Abstract

Immunohistochemical detection of antigen in lamb tissues naturally infected with sheeppox virus.

Abstract

An outbreak of sheep pox was diagnosed in 3 sheep flocks in Van province, Turkey between January and April 1998. Various tissue samples from 6 naturally infected lambs were examined using the streptavidin-biotin peroxidase complex method. Sheep pox viral antigen was detected in the cytoplasm of sheep pox cells and degenerated epithelial cells of the skin, lungs and digestive tract involving typical sheep pox lesions. Nuclear staining was also observed in some typically deformed nuclei of sheep pox cells. The immunostaining of sheep pox virus showed a correlation with the presence of sheep pox cells and degenerated epithelial cells resembling sheep pox cells. To confirm the presence of sheep pox virus in the skin and lung samples, direct electron microscopy was performed and sheep pox virus was only demonstrated in 2 skin samples.