Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Cryopreservation of an avian spirochete strain in liquid nitrogen.

Abstract

Serum samples were obtained from chickens with clinical signs of spirochaetosis, thought to be caused by Borrelia anserina. Sterilized glycerol (at a dilution of 1:2, v/v) or dimethyl sulfoxide (DMSO; 1:10 v/v) were added to the sera before storage in liquid nitrogen. After 15 months storage, infectivity tests were conducted in chickens. Compared with glycerol, DMSO was a superior cryoprotectant. All chickens inoculated with the DMSO sera showed spirochaetaemia within 48 h after inoculation.