Production and partial purification of Staphylococcus aureus alpha toxin.
A simple method for production and purification of alpha toxin antigens of S. aureus was developed. Alpha toxin production measured by haemolytic activity was the highest at 18 h after inoculation in yeast extract culture medium supplemented with thiamine, nicotinic acid and casamino acid. Alpha toxin was purified by ammonium sulfate precipitation (65%) and ultrafiltration. Molecular weight of the toxin was 33 kDa.