Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR.

Abstract

A nested multiplex polymerase chain reaction was developed for genotyping of bovine viral diarrhoea viruses (BVDVs). The assay could detect as little as 3 TCID50/ml and typed 42 out of 42 cell culture isolates. BVDV was also successfully typed, with or without RNA extraction, from all 27 whole-blood samples examined from 22 carriers or probable carriers and 5 experimentally infected cattle.