Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1.
The development of a polymerase chain reaction (PCR) and nested-PCR assays for the detection of porcine parvovirus (PPV) DNA is described. The primers were designed to a highly conserved region of the PPV genome which codes for NS-1. Comparisons between PCR and haemagglutination assays (HA) for PPV detection in serial ten-fold dilutions of supernatants of infected cell cultures showed that PCR could detect PPV genetic sequences at a dilution up to 106 greater than the last dilution in which HA could still detect viral antigens. The PCR and nested-PCR assays were successfully used to detect PPV DNA in clinical samples.