Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1.

Abstract

The development of a polymerase chain reaction (PCR) and nested-PCR assays for the detection of porcine parvovirus (PPV) DNA is described. The primers were designed to a highly conserved region of the PPV genome which codes for NS-1. Comparisons between PCR and haemagglutination assays (HA) for PPV detection in serial ten-fold dilutions of supernatants of infected cell cultures showed that PCR could detect PPV genetic sequences at a dilution up to 106 greater than the last dilution in which HA could still detect viral antigens. The PCR and nested-PCR assays were successfully used to detect PPV DNA in clinical samples.