Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Juvenile hormone regulation of HMG-R gene expression in the bark beetle Ips paraconfusus (Coleoptera: Scolytidae): implications for male aggregation pheromone biosynthesis.

Abstract

A 1.16 kb complementary DNA representing approximately one-third of I. paraconfususHMG-R (encoding 3-hydroxy-3-methylglutaryl-CoA reductase) was isolated by polymerase chain reaction and sequenced. The predicted translation product was 59 and 75% identical to the corresponding portion of HMG-R from Drosophila melanogaster and Blattella germanica, respectively. Northern blots showed that topical application of juvenile hormone III (JH III) increased HMG-R transcript levels in male thoraces in an apparent dose- and time-dependent manner. These data support the model that JH III raises HMG-R transcript levels, resulting in increased activity of the isoprenoid pathway and de novo pheromone production.