Pheromone precursor synthesis is localized in the metathorax of Ips paraconfusus Lanier (Coleoptera: Scolytidae).
By measuring the incorporation of [14C]acetate into the pheromone precursors ipsenone and ipsdienone in various tissues in vitro, it was demonstrated that pheromone precursor synthesis in Ips paraconfusus is closely associated with flight muscles in the metathorax. In addition, the role of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-R), one of the key regulatory enzymes of the isoprenoid pathway, was investigated by northern blot analyses of selected tissues. Northern blots revealed that HMG-R cDNA accumulation was localized to the thorax. The strategy for de novo pheromone production in I. paraconfusus is discussed.