Study on the application of reverse transcription polymerase chain reaction for the detection of the nucleic acid of infectious bronchitis virus.
A pair of specific primers were designed and synthesized using the nucleotide sequence of the S1 protein gene from Gray strain of infectious bronchitis virus (IBV). The cDNAs of the S1 protein gene of 3 IBV isolates from Nei Menggu, Tianjin and Shandong, China, were amplified by reverse transcription polymerase chain reaction (RT-PCR). The expected size, 1.0 kb, of cDNA fragments was obtained from the RNAs of the 3 isolates. The detection limit of the RT-PCR assay was 10 pg of RNA template. Results of restriction mapping analysis showed that there were one HaeIII, one TaqI and no MspI sites on the cDNA fragments from the 3 IBV isolates.