Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Insect-resistant transgenic brinjal plants.

Abstract

A synthetic cry1Ab gene encoding an insecticidal crystal protein of Bacillus thuringiensis (Bt) was transferred to brinjal (Solanum melongena cv. Pusa Purple Long) by cocultivating cotyledonary explants with Agrobacterium tumefaciens. Transformant plants resistant to kanamycin were regenerated. Hybridization experiments demonstrated gene integration and mRNA expression. Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants. The expression resulted in a significant insecticidal activity of transgenic brinjal fruits against the larvae of fruit borer (Leucinodes orbonalis). The results also demonstrated that a synthetic gene based on monocotyledon codon usage can be expressed in dicotyledonous plants for insect control.