Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Detection of cymbidium mosaic virus and odontoglossum ringspot virus by ELISA and RT-PCR from cultivated orchids in Korea.

Abstract

A study was carried out to detect cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) in cultivated orchids in Korea Republic. The standard DAS-ELISA and reverse transcription polymerase chain reaction (RT-PCR) were carried out for detection of the viruses in orchid samples. ELISA was suitable for massive-scale diagnosis of viruses in orchids. RT-PCR was a rapid, time-saving and reliable detection method, and detection limit data showed that RT-PCR was 103 times more sensitive than ELISA. Of the 321 individual orchids representing 5 orchid genera tested by ELISA, CymMV and ORSV were detected in 15.6% and 22.4%, respectively. Both viruses were found in 4.9% of orchids. Of the Cymbidium plants tested, cultivated plants showed 52.5% virus infection with either CymMV or ORSV or both viruses.