Isolation and identification of barley yellow mosaic virus in Korea.
Barley yellow mosaic bymovirus (BaYMV-HN) from Haenam, Korea Republic, was isolated by mechanical inoculation to barley cultivars, purification and production of antibodies. A filamentous virus, 13 nm in diameter and 250-300 nm and 500-650 nm in length, was isolated from infected plants. A specific antibody was made by injecting the purified virus into the muscle of a rabbit. In gel-diffusion tests, antibodies to BaYMV-HN did not make a spur with 2 Japanese BaYMV isolates (BaYMV-II-1 or BaYMV-III). BaYMV-HN resulted in yellowing and necrosis in host plants. Mechanical inoculation tests with Japanese barley cultivars showed that BaYMV-HN infected New Golden, Akagi Nijo and Tosan Kawa 73, but did not infect Amagi Nijo, Haruna Nijo, Ishukushirazu (ym3), Misato Golden (Ym1), Kashimamugi, Joshushiro Hadaka or Mokusekko 3 (ym1). In Korean barley cultivars, some of the naked barleys, including Hinssalbori, Kinssalbori, Saessalbori and Saechalssalbori, were not infected by BaYMV-HN. However, it infected all the covered barley cultivars and the beer barley cultivars. BaYMV-HN had 2 RNAs, RNA 1 (7.6 Kb) and RNA 2 (3.5 Kb), and 1 coat protein (33 KDa).