Characterization of an isolate of cucumber mosaic virus from forsythia (Forsythia koreana Nakai.).
An isolate of cucumber mosaic cucumovirus (CMV) was isolated from F. koreana [F. viridissima] showing mosaic symptoms. It was designated as CMV-Fk and some of its properties were characterized. CMV-Fk was distinct from CMV-Y by the appearance of symptoms such as mosaic, ring spot or line pattern on several tobacco plants, while CMV-Y used as a control virus induced yellow mosaic symptoms on the plants. Physical properties of CMV-Fk are as follows: thermal inactivation point was 55°C, dilution end point was 10-3, and longevity in vitro was 2-3 days. CMV-Fk was serologically identical to CMV-Y, and the virion was observed by EM as isometric particle of c. 30 nm in diameter. SDS-PAGE of the coat protein showed that CMV-Fk have one major protein band at the same migration site as CMV-Y. CMV-Fk consisted of 4 RNAs in genomic RNA or dsRNA analysis, but satellite RNA was not detected.