Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly Bemisia tabaci.

Abstract

Tomato yellow leaf curl bigeminivirus (TYLCV) was detected in the progeny of a single viruliferous whitefly (Bemisia tabaci) on aubergines and cotton by PCR amplification, Southern blot hybridization and transmission tests. The passage of TYLCV through the egg from the first to the second generation of viruliferous whitefly progeny was investigated using the same techniques. TYLCV DNA was present in all of the developmental stages of the whitefly progeny that had acquired the virus through the egg. When the adults developed from eggs laid on aubergines or cotton plants during a 5-day period by a single viruliferous whitefly were caged on tomato plants for 72 h, 10% of the tomato plants were infected. They produced similar symptoms to those observed in whitefly-mediated plant-to-plant inoculation. Eggs laid by viruliferous insects on artificial medium, ovaries and maturing eggs of viruliferous insects contained TYLCV DNA. It is concluded that TYLCV can be transmitted via the egg for at least 2 generations, and that the whitefly might serve as a reservoir for the virus in the absence of plant hosts between growing seasons.