Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses.
An immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) based assay has been developed for the detection of yam-infecting potyviruses. Based upon the same format 2 distinct simple tests were developed which allow the reliable diagnosis of yam mosaic potyvirus and the tentatively named yam mild mosaic virus infecting yams. By using immunocapture and a single-buffer RT-PCR reaction, the test can be performed in a single tube. The tests described have been shown to exhibit a 100-fold increase in detection sensitivity compared with existing ELISA assays.