Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Evaluation of dot-immunobinding assay and rapid immunofilter paper assay for detection of cymbidium mosaic virus in orchids.

Abstract

Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were evaluated for the detection of cymbidium mosaic potexvirus (CyMV) in orchids (Cattleya, Cymbidium, Dendrobium, Phalaenopsis and Vanda). The minimum detection levels of CyMV using these methods were 100 ng/ml in purified preparations, and CyMV was detected in a 10-4 dilution of extracts from infected orchid leaves. Although DIA took 5-6 hours for CyMV detection, it was reliable for diagnosis of a large-number of samples. RIPA enabled CyMV detection within a few minutes, and sensitivity was similar to that of DIA. It is suggested that RIPA is suitable as a rapid tool for diagnosing virus diseases in orchids. Using RIPA, CyMV and odontoglossum ringspot tobamovirus were detected simultaneously in extracts from a plant infected by both viruses.