Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. It is suggested that MAbAL is elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. It is concluded that MAbAL is a useful addition to the panel of PPV-specific MAbs available to date.