Cucumber mosaic virus, tobacco streak virus, and cucumber mosaic virus satellite RNA associated with mosaic and ringspot symptoms in Ajuga reptans in Ohio.
Commercial samples of creeping bugleweed (Ajuga reptans) cvs. Royalty, var. Atropurpurea Bronze, Bronze Beauty and Burgundy Glow showing mosaic and ringspot symptoms were tested for the presence of virus infection by direct antibody sandwich-ELISA and viral associated double stranded (ds) RNA analysis. Cucumber mosaic cucumovirus and alfalfa mosaic alfamovirus were detected by both techniques but tobacco streak ilarvirus was detected only by ELISA. dsRNA analysis also indicated the presence of a low molecular weight RNA associated with symptomatic and asymptomatic Royalty and var. Atropurpurea Bronze plants tested. Northern blot analysis confirmed that the low molecular weight RNA was cucumber mosaic cucumovirus satellite RNA. This is the first report of TSV, CMV and its satellite RNA in A. reptans in the USA.