Diagnosis of porcine cysticercosis: a comparative study of serological tests for detection of circulating antibody and viable parasites.
The performance of a monoclonal antibody-based (HP10) antigen capture assay was investigated in pigs experimentally infected with Taenia solium. Serum antibodies of the infected pigs were also measured by ELISA assays using 2 parasite preparations as antigens; T. solium and T crassiceps cyst fluid antigens bearing the HP10 epitope. Low-background values were obtained with sera from non-infected animals in all the assays used. In heavily infected pigs, both antigens and antibodies were detected as early as 29 days pi and remained detectable up to 200 days pi. In lightly infected pigs however, antigen and antibodies were first observed between 61-97 days pi. It is concluded that the HP10 antigen detection ELISA can be used to detect T. solium cysticercosis and that levels of serum antigen and antibody vary with the intensity of infection.