Invasive Species Compendium

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Abstract

Study on the origin of Populus tomentosa Carr. using the RAPD method.

Abstract

Five varieties of P. tomentosa and their putative parents (P. alba, P. davidiana [P. tremula var. davidiana], P. adenopoda and P. tremula) were analysed using RAPD [random amplified polymorphic DNA] and artificial crosses. Of 78 bands revealed using 13 arbitrarily selected primers, 58 (74.36%) bands were polymorphic among P. tomentosa and the 4 putative parents. The RAPD statistical results showed that the relationship between P. tomentosa and P. alba or P. adenopoda was closer than that between P. tomentosa and P. tremula var. davidiana or P. tremula. There were higher similarities and lower genetic distances between P. tomentosa and P. alba or P. adenopoda than that between P. tomentosa and the other 2 putative parents. The results from UPGMA (unweighted pair grouping by mathematical average) cluster analysis, and the Fitchmargoliash phylogenetic dendrogram were similar. In addition artificial hybrids including reciprocal crosses between P. alba and P. adenopoda were compared with 5 varieties of P. tomentosa. The hybrids between P. alba as female and P. adenopoda as male were closer to P. tomentosa than those from the reciprocal cross. The results demonstrated that P. tomentosa was a natural hybrid between P. alba female parent and P. adenopoda as male parent.