Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle.

Abstract

The nucleotide sequence for the coding region of the myostatin gene was determined for 32 double-muscled cattle sampled from 10 European cattle breeds. Seven DNA sequence polymorphisms were identified, of which 5 were predicted to disrupt the function of the protein, one was a conservative amino acid substitution, and one a silent DNA sequence variant. Four additional DNA sequence polymorphisms were identified in myostatin intronic sequences. In all but 2 breeds, all double-muscled animals were either homozygotes or compound heterozygotes for 1 of the 5 loss-of-function mutations. The absence of obvious loss-of-function mutations in the coding sequence of the 2 remaining breeds points either towards additional mutations in unexplored segments of the gene, or towards locus heterogeneity of double-muscling.