The antibody response of snakehead, Channa striata Bloch, to Aphanomyces invaderis.
Snakeheads (500g) were inoculated with microwaved spores of A. invaderis PA7 (5000 spores i.m.) and then microwaved PA7 spores mixed with homogenized mycelium (5000 spores mixed with 1 mat of mycelium i.p.) 10 days later, or inoculated with viable motile PA7 spores (5000 spores i.m.) or kept as unvaccinated, healthy controls. Pooled serum samples collected 30 days after inoculations were examined by Western immunoblotting. The SDS-PAGE protein profile of A. invaderis strain PA7, isolated from snakeheads during an outbreak of enzootic ulcerative syndrome (EUS) in Thailand, was similar to that of strain NJM9030 isolated from ayu during a mycotic granulomatosis infection in Japan and to strain 3P isolated from grey mullet infected with redspot disease in Australia. It is suggested strains NJM9030 and 3P are the same agent responsible for EUS in Southeast Asia.