Chromosomal locations of genes related to flavonoid and anthocyanin production in leaf sheath of shallot (Allium cepa L. Aggregatum group).
HPLC analyses using series of alien monosomic addition lines (AMALs) of Japanese bunching onion (Allium fistulosum) having extra chromosomes from shallot (A. cepa Aggregatum group) were performed to determine the chromosomal locations of the genes for flavonoid and anthocyanin production in leaf sheaths of A. cepa. In HPLC profiles both at 360 and 520 nm, several peaks were observed in A. cepa and in the AMAL with chromosome 5A from A. cepa, but no peak was observed in A. fistulosum or the other AMALs. Four of the compounds observed at 360 nm were identified as known flavonoids, i.e., apigenin, kaempferol, quercetin and rutin. Five out of the total 18 compounds at 520 nm were identified as known anthocyanins, i.e., cyanidin-3-glucoside, cyanidin-3-laminariobioside, peonidin-3-glucoside, cyanidin-3-malonylglucoside and cyanidin-3-malonyllaminariobioside. The results revealed that a group of the genes related to the flavonoid and anthocyanin production in the leaf sheath of A. cepa Aggregatum group are located on the chromosome 5A.