Identification and chromosomal assignment of RAPD markers linked with a gene for seed colour in a Brassica campestris-alboglabra addition line.
The present study is concerned with a Brassica campestris-alboglabra monosomic addition line (AA + 1 chromosome from C, 2n = 21) which harbours a B. alboglabra (a form of B. oleracea, CC, 2n = 18) chromosome with a gene controlling seed colour. Random amplified polymorphic DNA marker analysis revealed 19 markers specific to the alien C-genome chromosome of the addition line. One of these markers was inferred to be close to the seed colour gene. The location of the seed colour gene in a terminal region of the alien chromosome was deduced from what could have been homoeologous recombination or chromosomal deletion. Studies on mitotic prometaphase chromosomes of the addition line indicated that the alien chromosome was apparently chromosome 1 of B. alboglabra. The use of the present addition line is discussed in the context of breeding for yellow-seeded B. alboglabra and B. napus (AACC, 2n = 38).