A database for identification of Pseudomonas syringae pv. actinidiae, the pathogen of kiwifruit bacterial canker, using Biolog program.
Reactions of P. syringae pv. actinidiae to 95 carbon sources in a 96-well microplate (BiOLOG GN MicroPlateTM) were investigated. The bacterium used 9 carbon sources including D-mannitol and sucrose, but did not use 62 other carbon sources including α-cyclodextrin and dextrin. Based on these reactions, a user database for the identification of P. syringae pv. actinidiae was constructed in the Biolog programme (BiOLOG MicroLogTM 2 system). P. syringae pv. actinidiae isolates collected from kiwifruits could be identified automatically using the database, which could diagnose rapidly and easily whether the tree was infected with bacterial canker or not.