Invasive Species Compendium

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Abstract

Random amplified polymorphic DNA markers of the Brassica alboglabra chromosome of a B. campestris-alboglabra addition line.

Abstract

The alien C-genome chromosome in a B. campestris-B. alboglabra monosomic addition line was characterized by random amplified polymorphic DNA (RAPD) analysis. The alien chromosome carried 3 loci, Ec, Wc and Lap-1C, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine [cytosol] aminopeptidase (Lap-1Cc), respectively. The RAPD analysis revealed 17 markers specific to the alien chromosome. Among 45 offspring from the selfed addition line, the alien C-chromosome was transmitted to 15 plants, 4 plants had only parts of this chromosome and the remaining 26 plants did not carry the chromosome at all.