Factors affecting in vitro shoot proliferation and ex vitro establishment of mangosteen.
Shoot proliferation was achieved in mangosteen (Garcinia mangostana) using seed explants. The highest mean number of shoots per explant (16.8) was obtained from cultures on MS medium supplemented with 40 msmallcap˜M benzyladenine and 25 msmallcap˜M NAA, and these were maintained at 30°C with an 8-h photoperiod. Cultures on the same medium but supplemented with 2 g activated charcoal/litre produced fewer shoots. However, growth of these shoots was more organized and 75% rooting was obtained. Woody Plant Medium was not a suitable medium for shoot proliferation.