Invasive Species Compendium

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Abstract

Maternal microinjection of the endoparasitoid Cardiochiles diaphaniae (Hymenoptera: Braconidae).

Abstract

Maternal microinjection is the injection of DNA into an adult female to deliver DNA to the eggs. The adaptation of this method to the parasitoid Cardiochiles diaphaniae is reported. The results of preliminary dissections and injections with the plasmid pJKP2, followed by slot blot analysis, suggested that this method could be used to deliver injected DNA to the ovaries of this wasp. Subsequently, 16 adult females were injected and allowed to produce offspring (the G0 generation). Polymerase chain reaction analysis of the next (G1) generation revealed that several individuals contained the plasmid sequences. In a 2nd experiment, the plasmid phsopd, containing the parathion hydrolase gene (opd) of Pseudomonas diminuta, was injected. The plasmid probe hybridized to high molecular weight DNA from 3 of 38 G1 adults. A 4th adult produced a hybridization pattern consistent with integration of plasmid into the nuclear genome. The results indicate that maternal microinjection can result in transformation of this parasitoid.