Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Geographical origin of an introduced insect pest, Listronotus bonariensis (Kuschel), determined by RAPD analysis.

Abstract

Listronotus bonariensis is an important introduced pasture pest in New Zealand. Geographical populations of this species were analysed using polymerase chain reaction-based randomly amplified polymorphic DNA (RAPD), in an attempt to determine the geographical origin of the pest. Morphologically indistinguishable individuals were collected from 9 South American, 5 New Zealand and 1 Australian populations. Ten primers were screened for usefulness, 2 of which revealed significant, scorable polymorphisms between these populations. The result indicated that the sampled New Zealand L. bonariensis populations originated from the east coast of South America.