Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Detection of maize yellow stripe tenui-like virus by ELISA and dot-blot tests in host plants and leafhopper vector in Egypt.

Abstract

Antisera to the nucleoprotein of maize yellow stripe tenuivirus (MYSV) were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MTSV in naturally or experimentally infected maize, wheat, barley, oats and the graminaceous weeds Bromus wildenowii, Cenchrus biflorus, Dichanthium annulatum, Digitaria sanguinalis, Echinochloa colonum, Setaria verticillata and S. viridis. In maize leaves, differences in virus titre appeared to be correlated with leaf age and with MYSV symptom-types. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers (Cicadulina chinai).