Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Micropropagation of Acacia mangium Willd. through nodal bud culture.

Abstract

In vitro multiple shoot proliferation was achieved in A. mangium using nodal explants of an 8-yr-old elite tree. The maximum number of shoots were obtained on MS medium supplemented with BA ([benzyladenine] 3 mg/litre) and NAA (0.1 mg/litre). Addition of ascorbic acid (100 mg/litre) to the shoot proliferation medium inhibited browning of the medium. Shoots regenerated in vitro were rooted on MS medium supplemented with IBA (1 mg/litre) and IAA (0.5 mg/litre). Genotypically uniform regenerated plantlets survived (70%) in soil after hardening.