Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Improvements in non-precoated indirect enzyme-linked immunosorbent assay for specific detection of three potyviruses infecting cucurbitaceous plants.

Abstract

Improvements are described to a broadly specific, non-precoated indirect ELISA using cross-absorbed antisera to establish a simple method for the immunodiagnosis of watermelon mosaic 2 potyvirus (WM2V), zucchini yellow mosaic potyvirus (ZYMV) and papaya ringspot (type W) potyvirus (PRSV-W) in cucurbitaceous plants. ELISA using the pH 9.6-denatured homogenate of ZYMV-infected leaves to dilute antiserum against WM2V and the pH 9.6-denatured homogenate of WM2V-infected leaves to dilute antiserum against ZYMV were very effective in eliminating cross reaction between WM2V and ZYMV. When using these cross absorbed antisera against WM2V, ZYMV and PRSV-W, each virus could specifically be detected.