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Abstract

Nucleotide sequence analysis of a cDNA clone encoding the 34K movement protein gene of odontoglossum ringspot virus, ORSV-Cy, the Korean isolate.

Abstract

The partial nucleotide sequence of the 3′-terminal region of the Korean isolate of odontoglossum ringspot tobamovirus (ORSV-Cy) from cool-growing Cymbidium goeringii was determined. The sequence contained a full length open reading frame (ORF) coding for the viral cell-to-cell movement protein (MP). The ORF was located upstream of the coat protein gene and 105 nucleotides longer than that of tobacco mosaic tobamovirus (TMV). The ORF predicted a polypeptide chain of 303 amino acids with a molecular weight of 33 573. The ORF contained a similar region of conserved sequence motif of tobamoviruses and the putative assembly origin of the viral RNA was located at about 1100 nucleotides away from the 3′ end. The predicted amino acid sequence for the MP gene of ORSV-Cy was more closely related to pepper mild mottle tobamovirus, TMV-vulgare, TMV-Rakkyo than to tobacco mild green mosaic tobamovirus, TMV-L, cowpea strain of TMV [sunn-hemp mosaic tobamovirus] and cucumber green mottle mosaic tobamovirus.