Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses.

Abstract

The complete nucleotide sequence of RNA 1, the largest genomic segment of rice stripe tenuivirus (RSV), was determined using 2 sets of overlapping cDNA clones. RNA segment 1 comprises 8970 nucleotides and on the viral complementary sequence has a single long open reading frame coding for a protein of 2919 amino acids with an estimated Mr of 336 860. Amino acid sequence comparisons of the putative protein indicated strong homology (30% amino acid identity over c. 1500 residues) with the L protein of the genus Phlebovirus of the Bunyaviridae, but no detectable similarity with other members of the Bunyaviridae. However, weak similarity was detected with the L protein of Tacaribe arenavirus. The highly homologous sequence domain includes the conserved motifs of the putative RNA-dependent RNA polymerase. It is concluded that there are significant similarities in genome organization, structure and expression between RSV and members of the genus Phlebovirus of the Bunyaviridae. It is proposed that tenuiviruses should be included in the Bunyaviridae under the genus Tenuivirus.