Propagation of Bambusa vulgaris (yellow bamboo) through nodal bud culture.
Bambusa vulgaris (yellow bamboo), is the most commonly cultivated and used bamboo species in many countries. With the increased demand for bamboo, the importance of bamboo plantations has been realised. Establishment of such plantations would require large quantities of planting material on a continuous basis. Tissue culture techniques offer a solution to this problem. The propagation of B. vulgaris through nodal-bud culture was studied. Single nodal segments were tested for bud-break and shoot growth on basic Murashige and Skoog (MS) medium supplemented with different combinations and concentrations of growth regulators. Results suggest that cytokinin is important for bud-break, while gibberellic acid enhances multiple shoot production. The position of the node on the culm appears to affect bud-break and multiplication; middle nodes are the most suitable. Also, removal of prophylls enhances bud-break. Shoots developed from axillary buds could be rooted on MS basic medium at 50% macro[elements] and IBA (0.25 µsmallcap˜M). Upon transfer to the field (after four weeks in the rooting medium) the shoots developed into true-to-type plants.