Micropropagation of forest trees from Bangladesh.
Disinfected seeds of Leucaena leucocephala, Dalbergia sissoo and Albizzia procera [Albizia procera] were germinated in vitro to provide seedlings as explant sources for micropropagation. The organogenic capacity of segments of excised cotyledons, hypocotyls and roots, and of cotyledonary nodes, were tested on Murashige and Skoog and Woody Plant media supplied with different combinations of cytokinins and auxins. All three species developed shoots from cotyledonary buds. Such shoots could be used as further sources of nodes and shoot tips for continued axillary proliferation. Adventitious budding and shooting occurred on root and hypocotyl segments of A. procera only. Shoots of L. leucocephala rooted without special treatment at the end of the multiplication cycle; shoots of A. procera and D. sissoo necessitated a specific auxinic rooting medium, after a prior passage on a medium without growth regulators for A. procera. Plantlets of all three species were successfully acclimatized in soil in a greenhouse.