Evaluation in vitro and in vivo of a traditional antimalarial, "Malarial 5".
Malarial 5 is a traditional Mali drug composed of Cassia occidentalis leaves, Lippia chevalieri plants and Spilanthes oleracea flower heads, administered as a decoration. The preparation and its individual components were used in an in vitro experiment with Plasmodium falciparum and an in vivo assay with P. berghei in mice. The IC50 was 500 and 700 µg/ml for Malarial 5 and C. occidentalis, respectively; however, it was 200-400 µg/ml for the other components. In vivo, mice orally treated with 200 mg of lyophilized Malarial 5 over 5 days survived for 2-3 days longer than controls. To improve the antimalarial activity of Malarial 5 it is suggested that the proportions of L. chevalieri and S. oleracea, the more effective components, be increased.